For each level, the player must choose from three difficulty levels. Each level includes a single checkpoint in the middle where the player can restart if they die or fail to complete an objective (with the exception of the last level on any difficulty and the fourth level on easy). A few levels have secondary objectives, which are not required to complete unless on the normal or hard difficulty setting. Some objectives are present at the start of the level, while others are added during play. Each level is in a different time period and contains a series of objectives that must be completed. The main story mode of TimeSplitters 2 is divided into ten levels. It is possible to dual wield some weapons. Some weapons have an alternate fire which activates a feature such as launching a grenade or detonating a remote mine.

They are of many different time periods, from the historical to the futuristic. The weapons of TimeSplitters 2 include handguns, rifles, submachine guns, shotguns, rocket launchers, a grenade launcher, various explosives, a crossbow, a flamethrower, a fire extinguisher and a brick. Armour and health bars on the sides of the screen lower when the player is shot, which can be increased by walking over body armor and first aid kits. TimeSplitters 2 is a first-person shooter that requires players to kill enemies and complete objectives using a variety of weapons and tactics in different predefined scenarios.

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